WRN[C727A](Homozygous,AF%:100) Isogenic HCT116
CBP75081
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| I. Background | |
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| II. Description | |
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| III. Introduction | |
| Host Cell: | HCT116 |
| Expressed gene: | WRN[C727A] |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | McCoy's 5a +10%FBS |
| Storage: | Liquid nitrogen |
| IV. Representative Data | |
![]() Figure 1. Sanger of WRN[C727A](Homozygous,AF%:100) Isogenic HCT116 ![]() Figure 2. A:HCT116 Parental Cell;B:WRN[C727A](Homozygous,AF%:100) Isogenic HCT116 通道2 藍(lán)色微滴代表WRN[C727A]突變陽(yáng)性,通道2 綠色微滴代表該位點(diǎn)為陰性野生型 ![]() Figure 3. CTG Proliferation Assays of WRN[C727A](Homozygous,AF%:100) Isogenic HCT116(1C22). ![]() Figure 4. CTG Proliferation Assays of WRN[C727A](Homozygous,AF%:100) Isogenic HCT116(1C22). |
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